Part:BBa_M50082

Encodes PchB and PchA which allows for salicylic acid production in E. coli

Our composite part encodes a T5 promoter, strong RBS binding site, HIS tag, PchB and PchA proteins, Flag tag, and terminator. The PchB protein encoding region includes an RBS binding site for the PchA protein. While all of these parts are necessary for varying experimental observations, the overall goal of the part is to convert chorismate to salicylic acid in Escherichia coli. Once translated, the PchA enzyme converts chorismate to isochorismate. The PchB enzyme naturally catalyzes the conversion of isochorismate to salicylate and pyruvate. Both enzymes were originally found in Pseudomonas aeruginosa. The genes encoding PchB and PchA have been codon optimized for use in Escherichia coli by MIT's 2006 iGEM team, led by Andrew Endy. We include their part (BBa_J45017) in our composite part.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 524
  Illegal NgoMIV site found at 928
  Illegal NgoMIV site found at 1053
  Illegal NgoMIV site found at 1064
  Illegal NgoMIV site found at 1345
  Illegal NgoMIV site found at 1813
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 1995